Phage Display Synthetic Antibody Library

Antibodies are the first proteins which were successfully displayed on the surface of phage by fusing the coding sequence of scfv or fab to the coat protein.

Phage display synthetic antibody library. Antibody phage display has become an indispensable tool for the discovery and optimization of target specific monoclonal antibodies suitable for demanding applications including therapeutic reagents. Phage display technology has played a key role in the remarkable progress of discovering and optimizing antibodies for diverse applications particularly antibody based drugs. Several synthetic antibody phage display libraries have been created and used for the isolation of human monoclonal antibodies. Hucal platinum is a synthetic library of 45 billion fully human antibodies in fab format.

Antibody phage display library antibody libraries are constructed by the genomic information coding for antibody variable domains which can be derived from b cells of immune or naïve donors. Iontas management has a long track record of successfully generating human antibodies using phage display libraries and are recognised as experts in antibody discovery. Phage display of antibody libraries has become a powerful method for both studying the immune response as well as a method to rapidly select and evolve human antibodies for therapy. Monoclonal antibodies mabs are invaluable tools in biological research and have also proven effective as drugs.

The cat1 0 library cambridge antibody technology now part of medimmune astrazeneca is the first large naïve antibody phage display library. Construction of synthetic antibody phage display libraries springerlink. Antibody phage display was later used by carlos f. The in vitro nature of the technology enables the rapid and efficient identification of specific bin.

Barbas at the scripps research institute to create synthetic human antibody libraries a principle first patented. Iontas uses its proprietary technology to accelerate antibody drug discovery from target selection through to production and purification of fully human antibody for pre clinical use. It has been designed to cover the human antibody diversity to be flexible for further engineering and to yield antibodies with high expression titers. 1 while conventional hybridoma technologies still dominate as sources of antibody reagents phage display offers an attractive alternative for the production of mabs particularly for therapeutic uses which benefit from the use of human proteins.

The performance of antibody libraries which is usually measured in terms of their ability to yield high affinity binding specificities against target proteins of interest depends both on technical aspects such as library size and quality of cloning and on design. Rearranged antibody v genes for heavy and light chain genes were amplified from b cells of 43 human donors and randomly combined into scfvs resulting in a library with a size of 1 4 10 10.