Reagent Grade Water Specifications

Type 1 laboratory grade water is required for critical laboratory applications such as hplc mobile phase preparation blanks and sample dilution in gc hplc aa icp ms and other advanced analytical techniques.

Reagent grade water specifications. Generally reagent grade water means the distilled or de ionized water with no detectable amount of the compound or the element to be analysed at the detection limit of analytical procedure. The grade specifications specifically address contaminants of microbiological origin. Special requirements for reagent grade water distillation or equal process followed by ion exchange and 0 2 µ m filter distillation or equal process followed by ion exchange. Ro di distilled do not in themselves adequately define required water quality.

Feedwater to the final polishing step must have a maximum conductivity of 20 µs cm at 298k 25 c. Astm standards for laboratory reagent water astm d1193 91 astm. Feedwater to the final polishing step must have a maximum conductivity of 20 µs cm at 298k 25 o c. American society for testing and materials the astm standards are further subdivided into a b and c that can be used in conjunction with the type i ii iii or iv water above when bacteria levels need to be controlled.

Historically reagent water types i ii iii and iv have been linked to specific processes for their production. Reagent grade water rgw is water that is suitable for use in a specified procedure such that it does not interfere with the specificity accuracy and precision of the procedure. The most commonly used standards astm d1193 6 are summarized in the tables below. Preparation of buffers and culture media for mammalian cell culture and ivf.

International organization for standardization iso the iso based its specification on iso 3696 1987 and specifies three grades of water. Additional astm sub standards for laboratory reagent water. 1 2 1 type i grade of reagent water shall be prepared by distillation or other equal process followed by polishing with a mixed bed of ion exchange materials and a 0 2 µm membrane filter. Clinical and laboratory standards institute clinical laboratory reagent water.

Grade 1 grade 2 and grade 3 where grade 1 is the most pure see below. These specifications have been described by astm american socity for testing and materials d1193 iso international organization for standardization 3696 and clsi clrw. Process definitions alone e g. And preparation of solutions for electrophoresis and blotting.

1 2 2 type ii grade of reagent water shall be prepared by distillation using a still designed to produce a distillate having a conductivity of less than 1 0 µs cm at 298 k 25 c. Production of reagents for molecular biology applications dna sequencing pcr.