Rna Library Prep Illumina

Compatible with low quality degraded ffpe tissue.
Rna library prep illumina. This information is provided for use with illumina instruments only. Illumina library prep protocols accommodate a range of throughput needs from lower throughput protocols for small labs to fully automated library preparation workstations for large laboratories or genome centers. Download 1 mb. Jul 15 2020.
Benefit of panel modularity. Illumina stranded mrna prep is based on truseq ligation technology which has been cited in over 9 926 publications since 2011. In conjunction with ribosomal rna rrna depletion or poly a mrna enrichment. Revisionhistory document date descriptionofchange document 15004197 v02 june2016 inligateadaptersaboutreagentssection specifiedthatt4 rnaligase2.
This ligation method results in high coverage uniformity precise strand information and robust and reliable library preparation even from degraded samples. For the complete rna enrichment prep workflow you ll need to order library prep a probe panel and index adapters separately. Illumina stranded total rna prep supports a broad range of rna inputs from 25 ng to 1000 ng precise measurement of strand orientation. Rapid library preparation from a broad range of sample types for studying the coding and non coding transcriptome with unparalleled study flexibility.
Download 1 mb. Illumina total rna prep with ribo zero plus is based on truseq ligation technology which has been cited in over 9 926 publications since 2011. No mechanical shearing needed. A condensed version of the illumina stranded total rna prep ligation with ribo zero plus protocol for experienced users.
Oligonucleotide oligo sequences of illumina adapters used in library prep kits. Flexibility for many study designs. Illumina stranded total rna prep with ribo zero plus. Illumina rna prep with enrichment 25 off.
The ultra ii rna library prep kit for illumina is designed for non directional non strand specific rna library construction and delivers significantly increased sensitivity and specificity from your rna seq experiments from ever decreasing amounts of input rna. Exceptionally fast tagmentation based library prep enables deep insights into many transcripts of interest including the rna exome. Flexibility for many study designs.